Fig. 3: Demethylation of ZNF334 by TET1.
From: Targeted DNA demethylation of the ZNF334 promoter inhibits colorectal cancer growth
A The expression of TET1, TET2, and TET3 in HCoEpiC and CRC cells. Data are shown after normalization to β-actin (n = 3, *p < 0.05; **p < 0.01, compared with the HCoEpiC group). B TET1 mRNA expression in paired CRC tissues (n = 49, p < 0.001). C TET1 mRNA expression in HCT116 and RKO cells after pLVX-MYRF-TET1 infection. Data are shown after normalization to β-actin (n = 3, ***p < 0.001, compared with the blank group). D A CCK8 assay was performed to determine the growth of HCT116 and RKO cells transfected with pLVX-MYRF-TET1. (n = 3, **p < 0.01; ***p < 0.001, compared with the blank group). E ZNF334 mRNA expression of HCT116 and RKO cells transfected with pLVX-MYRF-TET1. Data are shown after normalization to β-actin (n = 3, **p < 0.01, compared with the blank group). The primer sequences are listed in Supplementary Table 5.
