Fig. 1: USP8 depletion decreases Wnt/β-catenin signaling activity in HCC cells. | Cell Death & Disease

Fig. 1: USP8 depletion decreases Wnt/β-catenin signaling activity in HCC cells.

From: USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization

Fig. 1

A The siRNAs specific to each deubiquitinating enzyme were transfected into LM3 cells. After 48 h, cells were lysed and the β-catenin protein level was analyzed by Western blot. B Western blot analysis of USP8 protein abundance in HCC cell lines and normal liver cells. C, D USP8 depletion decreased β-catenin protein level without affecting mRNA expression of β-catenin. E Immunofluorescence assay of USP8 and β-catenin. F Increasing amounts of USP8 WT or C786A were transfected into LM3 cells and β-catenin expression was detected. G, H USP8 depletion decreased β-catenin target genes using two different siRNA oligos. I USP8 depletion decreased TOP-luciferase activity. LM3 and HepG2 cells were transfected with SiUSP8 or SiControl together with TOP-luciferase reporter plasmid. Luciferase activity was measured 48 h after transfection. The results shown are representative of 3 independent experiments. Data are represented as mean ± SD of biological triplicates *P-value < 0.05; **P -value < 0.01; ***P-value < 0.001 by unpaired, 2-tailed Student’s t-tests.

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