Fig. 4: USP8 de-polyubiquitylates β-catenin. | Cell Death & Disease

Fig. 4: USP8 de-polyubiquitylates β-catenin.

From: USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization

Fig. 4

A LM3 cells transfected with the indicated siRNA were treated with MG132 for 6 h before collection. β-catenin was immunoprecipitated with anti-β-catenin and immunoblotted with anti-HA. B Immunoblotting was used to detect the ubiquitination of β-catenin in 293 T cells co-transfected with Myc-β-catenin, HA-Ubiquitin, and Flag-USP8 (wild type or C786A). C USP8 removed the ubiquitin chain of β-catenin in a dose-dependent manner. D HA-WT, K6, K11, K27, K29, K33, K48 or K63 Ub were co-transfected with Myc-β-catenin and Flag-USP8 into HEK293T cells. After treatment with 10 μM MG132 for 6 h, cell lysates were subjected to ubiquitination assay and the ubiquitination level of β-catenin detected by HA antibody.

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