Fig. 5: USP8 depletion inhibits HCC cell proliferation, invasion stem-like properties and promotes ferroptosis. | Cell Death & Disease

Fig. 5: USP8 depletion inhibits HCC cell proliferation, invasion stem-like properties and promotes ferroptosis.

From: USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization

Fig. 5

A USP8 depletion inhibited HCC proliferation. B USP8 depletion decreased clone formation capability of HCC cells. C, D Representative images of EdU assay of HCC cells. E Tranwell invasion assay of HCC cells. F USP8 depletion decreased sphere formation of HCC cells. G CCK8 assay was used to analyze the responses of HCC cells to erastin. H CCK8 assay showing the response of LM3 and HepG2 cell lines to erastin (20 μM)/± ferrostatin-1 (1 μM). I Lipid ROS, (J). ferrous iron levels, (K) and GSH levels were measured in LM3 cells and HepG2 cells. L USP8 depletion inhibits the tumor growth in vivo. LM3 cells were stably transfected with lentivirus carrying a scrambled shRNA or USP8 shRNA. 1 × 106 LM3 cells were injected to the right dorsal flank of each mouse. Tumor sizes were measured every 3 days until the end of the experiment. The results shown are representative of 3 independent experiments. Data are represented as mean ± SD of biological triplicates *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001 by unpaired, 2-tailed Student’s t-tests.

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