Fig. 2: Generation and validation of Gpatch2−/− mice.
From: Deletion of Gpatch2 does not alter Tnf expression in mice
A Gpatch2 primers and genotyping. Schematic representation of primers located in the introns flanking exon 3 of Gpatch2 for CRISPR-targeted deletion of exon 3 (blue) and genotyping (purple) to distinguish wild-type and Gpatch2 mutant alleles. B Exon 3 was deleted by CRISPR/Cas9 to cause a frameshift in Gpatch2−/− mice. C Immunoblotting for GPATCH2 in lysates from the testis of wild-type and Gpatch2−/− mice with hybridoma supernatant. D Observed numbers of wild-type, heterozygous Gpatch2+/−, and homozygous Gpatch2−/− offspring obtained from inter-crosses of Gpatch2+/− mice.
