Fig. 2: Ablation of SCAP in VSMCs impairs VSMC proliferation in mouse embryos.
From: SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in mice
A Immunohistochemistry analysis of α-SMA in the embryonic aorta at E14.5 (n = 6). Scale bar = 5 μm. B Statistical analysis of relative α-SMA-positive areas (n = 6). C Immunohistochemistry analysis of PCNA in embryonic aortas at E14.5 (n = 6). Scale bar = 5 μm. D Statistical analysis of relative PCNA-positive cells (n = 6). E Immunofluorescence staining of Ki67 in the embryonic aorta at E14.5 (n = 6). Scale bar = 5 μm. F Statistical analysis of relative Ki67-positive cells (n = 6). G Representative images of TUNEL staining of embryonic aortas at E14.5 in each group (n = 6). Scale bar = 25 μm. H Quantification of relative TUNEL-positive areas in each group (n = 6). I Immunofluorescence staining of CASPASE-3 in the embryonic aorta at E14.5 (n = 6). Scale bar = 5 μm. J Statistical analysis of the relative number of CASPASE-3 positive cells (n = 6). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were calculated by Student’s t test.
