Fig. 7: BSA-NP/si-Nodal nanocomplexes inhibit the proliferation and metastasis of CRC. | Cell Death & Disease

Fig. 7: BSA-NP/si-Nodal nanocomplexes inhibit the proliferation and metastasis of CRC.

From: Nodal promotes colorectal cancer survival and metastasis through regulating SCD1-mediated ferroptosis resistance

Fig. 7

A Schematic diagram for the preparation of BSA-NP/si-Nodal nanocomplexes. B Agarose gel retardation assay was performed to evaluate the encapsulation efficiency of BSA-NPs. C Transmission electron microscopic images of BSA-NP/si-Nodal nanocomplexes. D, E The hydrodynamic particle size and zeta potential distribution of BSA-NP/si-Nodal complexes were measured via dynamic light scattering. F Western blot analysis of Nodal, SCD1, Smad2/3, p-Smad2/3, E-cadherin, N-cadherin and snail expressions in cells with different treatments. G Schematic diagram of in vivo experiments in tumour-bearing mice models. H–J Tumour images, tumour volume and tumour weight in mice. K IHC analysis of the Ki67 and Nodal expressions. L Fluorescence images and fluorescence intensity analysis of nude mice with lung metastasis (∗∗P < 0.01 and ∗∗∗P < 0.001).

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