Fig. 1: Characterization of miR-122-5p mediated p53 effects in A549 cells.
From: MiR-122-5p regulates the mevalonate pathway by targeting p53 in non-small cell lung cancer
A The proliferation of A549 cells treated with 122-5p inh. combined with si-p53 or 122-5p inh. alone or 122-5p mim. combined with Nutlin-3 or 122-5p mim. alone using the CCK-8 assay. The colony formation assay could detect the long-term proliferation ability of A549 cells treated with 122-5p inh. combined with si-p53 or 122-5p inh. alone or 122-5p mim. combined with Nutlin-3 or 122-5p mim. alone (B) and the bar graph corresponding to B (D). The migration ability of A549 cells treated with 122-5p inh. combined with si-p53 or 122-5p inh. alone or 122-5p mim. combined with Nutlin-3 or 122-5p mim. alone using the cell scratch assay (C) and the bar graph corresponding to C (E). The apoptosis of A549 cells treated with 122-5p inh. combined with si-p53 or 122-5p inh. alone or 122-5p mim. combined with Nutlin-3 or 122-5p mim. alone using flow cytometry (F) and the analysis of early apoptosis (G) and late apoptosis (H). The above bar graphs were the sum of three independent experiments (mean ± SD). The ctrl group was represented by A549 cells treated using a medium solution containing 122-5p inh. NC and 122-5p mim. NC and si-p53 NC and DMSO, ns: not significant, *P < 0.05 and **P < 0.01 vs. Ctrl group, #P < 0.05 and ##P < 0.01 vs. 122-5p inh. group, &P < 0.05 and &&P < 0.01 vs. 122-5p mim. group.
