Fig. 2: Effect of p53 on the MVA pathway in A549 cells.
From: MiR-122-5p regulates the mevalonate pathway by targeting p53 in non-small cell lung cancer
A549 cells treated with si-p53 or Nutlin-3, RT-qPCR could detect the relative mRNA expression of p53 (A) and ABCA1 (B). p53, ABCA1, SREBP2(P), SREBP2(M) protein expression using western blotting (C) and the bar graph corresponding to C after statistical analysis (D) in A549 cells treated with si-p53 or Nutlin-3. E The relative mRNA expression of the MVA pathway genes HMGCS1 (F), HMGCR (G), MVK (H), PMVK (I), MVD (J), FDPS (K), FDFT1 (L), SQLE (M), and GGPS1 (N) by RT-qPCR in A549 cells treated with Nutlin-3 combined with DL-MVA or Nutlin-3 alone or DL-MVA alone. The expression of the MVA pathway key proteins HMGCS1, HMGCR, and FDFT1 using western blotting (O) and the bar graph corresponding to O (P) in A549 cells treated with Nutlin-3 combined with DL-MVA or Nutlin-3 alone or DL-MVA alone. The above bar graphs were the sum of 3 independent experiments (mean ± SD). The ctrl group was A549 cells treated with a medium solution containing si-p53 NC and DMSO, ns: not significant, *P < 0.05 and **P < 0.01 vs. Ctrl or DMSO group, #P < 0.05 and ##P < 0.01 vs. DL-MVA group.
