Fig. 6: CMTR1 knockdown enhances the efficacy of PD1 blockade immunotherapy in colorectal cancer. | Cell Death & Disease

Fig. 6: CMTR1 knockdown enhances the efficacy of PD1 blockade immunotherapy in colorectal cancer.

From: CMTR1 promotes colorectal cancer cell growth and immune evasion by transcriptionally regulating STAT3

Fig. 6

A Schematic diagram showing the time point of anti-PD1 antibody treatment and analysis of tissues from C57BL/6 J mice implanted with MC38 cells. B, C After the knockdown of CMTR1 expression using shRNA, the expression of CMTR1 was measured by RT‒qPCR and western blotting. D, E Representative pictures of MC38 cell-derived tumors in C57BL/6 J mice, and tumor weights indicating the effects of CMTR1 knockdown or in combination with PD1. F, G Immunohistochemical staining and semi-quantitative analysis of CD8 positive T cells in tumors from mice indicating the effects of CMTR1 knockdown alone or in combination with PD1 blockade therapy. Scale bar: 50 µm. H, I Immunofluorescent staining and quantification of ki67 in tumors from mice upon CMTR1 knockdown or in combination with PD1. Scale bar: 50 µm. J–M Relative mRNA levels of inflammatory mediators in tumors from mice treated as indicated. *P < 0.05, **P < 0.01, and ***P < 0.001.

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