Fig. 3: Reduced METTL3 decreases cell proliferation and migration by regulating the ERK pathway. | Cell Death & Disease

Fig. 3: Reduced METTL3 decreases cell proliferation and migration by regulating the ERK pathway.

From: Post-transcriptional modification of m6A methylase METTL3 regulates ERK-induced androgen-deprived treatment resistance prostate cancer

Fig. 3

A The RNA levels of METTL3, HRAS and MEK2 were detected in LNcap-AI and C4-2 cells respectively transfected with sh-con and sh-METTL3 by RT-qPCR, and GAPDH was used as an internal reference. B The m6A levels were detected in 4 cell lines (LNcap-AI and C4-2transfected with sh-con, sh-METTL3, respectively) by dot-blotting. C The protein levels of METTL3, HRAS and MEK2 and phosphorylation of ERK1/2 were detected in 4 cell lines (LNcap-AI and C4-2 respectively transfected with sh-con, sh-METTL3) by Western blotting, and GAPDH was used as internal reference. D An MTT assay was used to detect the changes in cell viability after METTL3 knockdown. E The changes in cell proliferation after METTL3 knockdown were detected by plate cloning assay. Statistical results (right side) of the above proliferation assay. F Transwell assays were used to detect the changes in cell migration and invasion after METTL3 knockdown. G, H After overexpression of HRAS and MEK2 in LNCap-AI sh-METTL3 cells, the changes in RNA (G) and protein levels (H) were detected to determine knockdown efficiency. I After overexpression of HRAS and MEK2 in LNCap-AI sh-METTL3 cells, the changes in cell viability were observed by MTT assay.

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