Fig. 3: URB597 combined with RSL3 significantly inhibits the growth of RCC cells by inducing ferroptosis and cell cycle arrest. | Cell Death & Disease

Fig. 3: URB597 combined with RSL3 significantly inhibits the growth of RCC cells by inducing ferroptosis and cell cycle arrest.

From: Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway

Fig. 3: URB597 combined with RSL3 significantly inhibits the growth of RCC cells by inducing ferroptosis and cell cycle arrest.The alternative text for this image may have been generated using AI.

Level of lipid peroxidation (A), MDA (B), 4-HNE (C), and cytosolic ROS production (D) in 786-O and Caki-1 cells treated with URB597 (10 µM) and RSL3 (0.5 µM) singly or in combination for 48 h. Lipid peroxidation and cytosolic ROS were assessed by flow cytometry with C11-BODIPY and H2DCFDA, respectively. Histograms show the production of MDA, the production of 4-HNE, and the relative fold change in lipid ROS and cytosolic ROS (right panel). E Cell cycle distributions of 786-O and Caki-1 cells treated as in (A). The cell cycle was evaluated by flow cytometry after staining with propidium iodide (PI). Histograms show the cell cycle distributions of treated 786-O and Caki-1 cells (right). Data are means ± SDs of measurements repeated three times with similar results. **p < 0.01 versus the corresponding control (t test).

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