Fig. 4: FAAH substrates increase the sensitivity of RCC to ferroptosis inducers.
A Dose–response curves for RSL3 as a single agent or combined with AEA, PEA, and OEA at the indicated doses in 786-O and Caki-1 cells for 72 h. Viability was measured 72 h after treatment. Effects on cell viability were calculated as the percentage of vehicle-treated cells. B In the colony formation assay, 786-O and Caki-1 cells were treated as in (A) for 14 days, and cells were fixed and stained with crystal violet. C–E In the wound-healing assay, 786-O (C) and Caki-1 (D) cells were treated as in (A) for the indicated time. Histograms show the relative cell migration (E). Scale bar: 100 µm. Level of lipid peroxidation (F) and cytosolic ROS production (G) in 786-O cells treated as in (A) for 48 h. Lipid peroxidation and cytosolic ROS were assessed by flow cytometry with C11-BODIPY and H2DCFDA, respectively. Histograms show the relative fold change in the production of lipid ROS and cytosolic ROS (right panel). Data are means ± SDs of measurements repeated three times with similar results. ns (not significant), **p < 0.01 (one-way ANOVA).
