Fig. 6: RNA-Seq reveals inhibition of FAAH-regulated sensitivity to ferroptosis via the PI3K-AKT pathway.

A KEGG pathway analysis of downregulated differentially expressed genes in transcriptomes of 786-O cells after combinational treatment (Comb) with URB597 (10 µM) and RSL3 (0.5 µM). The 10 most significantly activated pathways are shown. B Heat map of significantly regulated genes of transcriptomes of 786-O cells after combinational treatment with URB597 (10 µM) and RSL3 (0.5 µM) correlated with the PI3K-AKT pathway (n = 3). C qPCR analysis of the indicated gene expression associated with the PI3K-AKT pathway in 786-O cells treated with URB597 (10 µM) and RSL3 (0.5 µM) singly or in combination for 48 h compared to parental 786-O cells. **p < 0.01 versus the corresponding control (t test). D The viability of 786-O cells stably transfected with empty vector or FAAH treated with RSL3 (0.5 µM), LY294002 (5 µM), or PD98059 (10 µM) singly or in combination. Cell viability was assessed by CCK-8 assay after treatment for 72 h with the indicated doses of drugs. Effect of FAAH overexpression, LY294002 (5 µM), and PD98059 (10 µM) on lipid peroxidation (E) and MDA (F) response to RSL3 treatment in 786-O cells. Data are means ± SDs of measurements repeated three times with similar results. ns (not significant), **p < 0.01 (one-way ANOVA).