Fig. 8: NSUN2-mediated m5C modification upregulates the expression of NTN1. | Cell Death & Disease

Fig. 8: NSUN2-mediated m5C modification upregulates the expression of NTN1.

From: Long noncoding RNA DIAPH2-AS1 promotes neural invasion of gastric cancer via stabilizing NSUN2 to enhance the m5C modification of NTN1

Fig. 8

a, b NTN1 mRNA and protein levels of designated HGC-27 and AGS cells were detected by qRT-PCR and western blot. c The degradation rate of NTN1 mRNA was calculated by qRT-PCR using indicated HGC-27 and AGS cells. Designated cells were treated with ActD (Actinomycin D, 2 mg/ml) for the indicated periods. d, e NTN1 mRNA and protein levels were detected by qRT-PCR and western blot in HGC-27 cells transfected with WT NSUN2, C271A/C321A NSUN2, or empty vector. f The degradation rate of NTN1 mRNA was measured utilizing HGC-27 cells transfected with WT NSUN2, C271A/C321A NSUN2, or empty vector as mentioned in (d) and (e). g Transwell assay including migration and invasion was performed utilizing indicated engineered HGC-27 cells. Scale bar: 100 μm. h Statistical graphs of the Transwell assay mentioned in (g) were exhibited. i The schematic diagram (created by Figdraw) depicting the mechanism by which the interaction between DIAPH2-AS1 and NSUN2 promotes GC-NI through NTN1 is shown. Data and error bars were shown as mean ± SD of triplicate independent replicate experiments and all data were analyzed by Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001).

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