Fig. 4: EZH2 conditional knockout upregulates the expression of PTEN, thus blocking the activation of EGFR/ERK1/2/STAT3 signaling pathway in I/R induced AKI-to-CKD transition mouse model. | Cell Death & Disease

Fig. 4: EZH2 conditional knockout upregulates the expression of PTEN, thus blocking the activation of EGFR/ERK1/2/STAT3 signaling pathway in I/R induced AKI-to-CKD transition mouse model.

From: Enhancer of zeste homolog 2 promotes renal fibrosis after acute kidney injury by inducing epithelial-mesenchymal transition and activation of M2 macrophage polarization

Fig. 4

A Kidney tissue lysates from I/R mice were prepared and subjected to immunoblotting analysis with antibodies against PTEN, p-EGFR, EGFR, and GAPDH. B–D Expression levels of PTEN, p-EGFR, EGFR in different groups were quantified by densitometry and normalized with GAPDH and EGFR respectively. E Photomicrographs showed the immunofluorescent staining of PTEN in different groups. F Kidney tissue lysates from I/R mice were prepared and subjected to immunoblotting analysis with antibodies against p-ERK1/2, ERK1/2, p-STAT3, STAT3, and GAPDH. G–J Expression levels of p-ERK1/2, ERK1/2, p-STAT3, STAT3 in different groups were quantified by densitometry and normalized with GAPDH, ERK1/2, and STAT3, respectively. K Kidney tissue lysates from I/R mice were prepared and subjected to immunoblotting analysis with antibodies against Snail, H3pSer10, and GAPDH. L, M Expression levels of Snail and H3pSer10 in different groups were quantified by densitometry and normalized with GAPDH. Data were expressed as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. N.S., statistically not significant, with the comparisons labeled. All scale bars = 50 μm.

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