Fig. 6: Hypoxia increases the expression of EZH2, which reduces the expression of FBXL7 and promotes PFKFB4 protein stability. | Cell Death & Disease

Fig. 6: Hypoxia increases the expression of EZH2, which reduces the expression of FBXL7 and promotes PFKFB4 protein stability.

From: Hypoxia-mediated promotion of glucose metabolism in non-small cell lung cancer correlates with activation of the EZH2/FBXL7/PFKFB4 axis

Fig. 6

A Western blot analysis showing elevated expression of EZH2 and HIF-1α proteins in hypoxic A549 cells. B Verification of the binding of HIF-1α to the EZH2 promoter region, as evaluated by dual-luciferase reporter assay. C Enrichment of HIF-1α in the promoter region of EZH2, as observed by ChIP assay. D mRNA expression of EZH2, FBXL7, and PFKFB4 in normoxic or hypoxic A549 cells in the presence of si-EZH2. E Western blot analysis showing the expression of EZH2, FBXL7, and PFKFB4 proteins in normoxic or hypoxic A549 cells in the presence of si-EZH2. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns p > 0.05. The cell experiment was repeated three times independently.

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