Table 1 Summary of experimental data and classification of p63 DBD mutations.

Mutation	Syndrome	Position	Activity	DNA binding	Zinc loss	Destabilization	Classification
S272N	ELA	L3	−				DNA contact
R279H	ELA	L3	−		−	−
R279C	ELA	L3	−
R279Q	ELA	L3	−
R304W	ELA	L3	−	−	+	−
R304Q	ELA	L3	−		+	−
R304P	ELA	L3	−		++	+
C308S	ELA	S10/H2	+	+++
C308Y	ELA	S10/H2	+	−
A315E	ELA	H2	−
Y192C	ELA	S4	+	+	++	+++	Zinc region
Y192D	ELA	S4	−
K193E	SHFM4	S4/Ha	+	++	+	+
V202M	ELA	L2A	+	+	++	+++
R204W	ELA	L2A	−	−	+++	+++
R204Q	ELA	L2A	+	+	++	+++
R204L	ELA	L2A	+	+	++	+
H208Y	ELA	H1	−		+++	+++
C269Y	ELA	L3	−
C273Y	ELA	L3	−
R280S	ELA	L3	−	−	+	+
R280C	ELA	L3	−	−	+	+++
R280H	ELA	L3	+	++	+	+++
154InsP	ELA	S2	+				H2 region
L162P	ELA	S2’	−
Y163C	ELA	S2’	−
C306Y	ELA	S10/H2	−
C306R	ELA	S10/H2	−
P309S	ELA	H2	−
D312G	ELA	H2	−
D312N	ELA	H2	−
D312H	ELA	H2	−
R313G	ELA	H2	−	−	+	+
G134D	ELA	N-term.	+++	+++			Dimer interface
K194E	SHFM4	S4/Ha	+	−	+	−
R227Q	ELA	S5	+	++	−	−
R298Q	ELA	S10	+++	++	−	+
R298G	ELA	S10	+++	++

The table shows all p63 DBD mutations examined in this study with the associated and summarizes the most important experimentally determined parameters together with the resultant classification (see discussion). The position of the mutated residue is annotated in relation to the secondary structure elements of the DBD (H α-helix, S β-strand, L loop; see Supplementary Fig. S1A for details). The results of the luciferase reporter assays are displayed in the column ‘Activity’ (-: inactive; +: wildtype-like; +++: hyperactive). Data from the DNA pulldown assay, the luciferase reporter displacement assay and SPR measurements are integrated in the column ‘DNA binding’ (-: no DNA binding; +: residual DNA binding; ++: moderate DNA binding; +++: wildtype-like DNA binding). The tendency of the mutant DBDs to lose the zinc ion as a measurement for the destabilization of the zinc finger is stated in the column ‘Zinc loss’ (-: no zinc loss; +: minor zinc loss; ++: moderate zinc loss; +++: strong zinc loss). The destabilization of the mutant DBDs is displayed in the column ‘Destabilization’ (-: no destabilization or stabilization; +: moderate destabilization; +++: strong destabilization).

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