Fig. 4: CHST2 is involved in MECA79 antigen synthesis to mediate breast cancer metastasis.

A FACS analysis of MDA-MB-231-Vector (black) and MDA-MB-231-CHST2 (red) cells stained by MECA79 mAb, left panel: Representative histograms were shown. Right panel: MFI of MECA79 antigen synthesis by breast cancer cells was shown compared with vector group, MFI, mean fluorescence intensity, the MFI of MECA79 less the MFI of isotype control equals the Delta MFI. Data were shown as mean ± SD, ****P < 0.0001, Student t test. B Flow cytometry analysis of the MECA79 surface expression in MCF-10A-Vector (black), CHST2 (red) or mutant CHST2-N475A (blue) cells, left panel: Representative histograms were shown. Right panel: Relative MFI of MECA79 antigen synthesis by MCF-10A cells was shown compared with the vector group. Data were shown as mean ± SD from three independent experiments. ****P < 0.0001, **P < 0.01, Student t test. C Flow cytometry analysis of the MECA79 surface expression in MCF-10A-CHST2 cells with treatment of 0, 5, 10 mM sodium chlorate for 48 h, left panel: Representative histograms were shown. Right panel: MFI of MECA79 antigen synthesis by MCF-10A-CHST2 cells was shown. Data were shown as mean ± SD from three independent experiments. ****P < 0.0001, Student t test. D H&E staining and Immunohistochemical staining of MECA79 antigen of the lung sections isolated from lung metastatic mice models. E, F Transwell assays in MDA-MB-231-Vector or MCF-10A-Vector and MDA-MB-231-CHST2 or MCF-10A -CHST2 cells with MECA79 or isotype antibody treated (left panel), Six fields chosen randomly were counted for statistical analysis (right panel) and data were shown as mean ± SD, ***P < 0.001, **P < 0.01. Student t test.