Fig. 5: KRT7-AS enhances cisplatin-induced apoptosis and activates apoptotic signaling pathways in cancer cells. | Cell Death & Disease

Fig. 5: KRT7-AS enhances cisplatin-induced apoptosis and activates apoptotic signaling pathways in cancer cells.

From: The long non-coding RNA keratin-7 antisense acts as a new tumor suppressor to inhibit tumorigenesis and enhance apoptosis in lung and breast cancers

Fig. 5: KRT7-AS enhances cisplatin-induced apoptosis and activates apoptotic signaling pathways in cancer cells.The alternative text for this image may have been generated using AI.

TUNEL assay showed that KRT7-AS enhanced cisplatin-induced apoptosis in SPC-A-1 (A) and H1299 (B) lung cancer cells, whereas, silencing of KRT7-AS in A549 (C) lung cancer cells reduced apoptosis. The levels of Caspase 3, cleaved-Caspase 3, BCL-2, and PARP in KRT7-AS-overexpressed SPC-A-1 (D) and H1299 (E) lung cancer cells, or KRT7-AS-silenced A549 (F) lung cancer cells were measured by Western blotting. Time course study showed that KRT7-AS elevated the levels of cleaved-Caspase3 and cleaved PARP in cisplatin concentration- and time-dependent manner (G–L). Data are shown as mean ± SD of three independent replicates. *P < 0.05, **P < 0.01.

Back to article page