Fig. 3: MBD2 regulates DDB2 expression in A549 cells on TGF-β1 stimulation.
From: MBD2 facilitates tumor metastasis by mitigating DDB2 expression
A Scatter plot showing the average gene expression level (log10) in A549 cells upon TGF-β1 stimulation vs the fold change (log2). The blue and red dots represent downregulated and upregulated genes, respectively. B KEGG pathway enrichment analysis of the upregulated DEGs. C Heatmap showing the upregulated P53-related genes in TGF-β1-treated A549 cells transfected with MBD2 siRNA. D RT-PCR analysis of MBD2, TP53 and DDB2 expression in TGF-β1 induced A549 cells after MBD2 siRNA transfected. E Global DNA methylation level in A549 cells following TGF-β1 treatment. F Results of the bisulfite DNA sequencing analysis of the DDB2 promoter in TGF-β1 induced A549 cells following MBD2 siRNA transfected. G ChIP-PCR analysis of MBD2 binding to the DDB2 promoter. H Relative luciferase activity in A549 cells after WT or MU plasmid transfected. I RT-PCR analysis of CDH1, CDH2 and VIM expression in A549 cells. J Comparison of DDB2 mRNA expression between lung adenocarcinoma tissue samples and adjacent nontumor tissue samples in the TCGA-LUAD cohort. K Comparison of DDB2 mRNA expression between premetastatic and metastatic tissues in the TCGA melanoma cohort. The data are presented as the mean ± SEM. GO Gene Ontology, DEG differentially expressed gene, KEGG Kyoto Encyclopedia of Genes and Genomes. *p < 0.05, **p < 0.01, ***p < 0.001.
