Fig. 4: MBD2 siRNA-loaded liposomes inhibited tumor metastasis in vitro.
From: MBD2 facilitates tumor metastasis by mitigating DDB2 expression
MIF in A549 (A) and B16F10 (B) cells transfected with DiI-labeled liposomes. C, D Western blot analysis of MBD2 expression in A549 (C) and B16F10 (D) cells after L-Scr siRNA or L-MBD2 siRNA transfected. E, F The migration ability of A549 (E) and B16F10 (F) cells transfected with L-Scr siRNA or L-MBD2 siRNA. G-H The invasion ability of A549 (G) and B16F10 (H) cells transfected with L-Scr siRNA or L-MBD2 siRNA. I, J Coimmunostaining of liposomes (Red) and E-cadherin (Green) in A549 (I) and B16F10 (J) cells after TGF-β1 stimulation. K, L Coimmunostaining of liposomes (Red) and Vimentin (Green) in A549 (K) and B16F10 (L) cells after TGF-β1 stimulation. All images were acquired at ×200 magnification. Nuclei were stained with DAPI (blue). M, N RT-PCR analysis of CDH1, CDH2 and VIM in TGF-β1 induced A549 (M) and B16F10 (N) cells following L-Scr siRNA or L-MBD2 siRNA treatment. MFI mean fluorescence intensity, L-Scr siRNA liposome-based Scrambled siRNA, L-MBD2 siRNA liposome-based MBD2 siRNA. The data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
