Table 1 Key resources table.

From: Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cells

 

Primary Antibodies

S. No.

Name

Company

Catalogue No.

1

Anti-Mitofusin 2

Abcam

ab56889

2

Recombinant Anti-DRP1

Abcam

ab184247

3

Anti-PGC1 alpha

Abcam

ab54481

4

Anti-MIRO1

Abcam

ab83779

5

Anti-LC3B

Abcam

ab48394

6

Anti-TOMM20

Abcam

ab56783

7

Anti-LAMP1

Abcam

ab25245

8

Recombinant Anti-Beclin 1

Abcam

ab207612

9

Anti-VDAC1/Porin

Abcam

ab15895

10

Anti-PGC1 alpha

Abcam

ab106814

11

Anti-PINK1

Abcam

ab75487

12

Anti-COX IV

Abcam

ab14744

13

Anti-FUNDC1

Abcam

ab74834

14

Anti-DRP1

Abcam

ab140494

15

Anti-SQSTM1/p62

Abcam

ab56416

16

Anti-Uteroglobin (CCSP)

Abcam

ab40873

17

Recombinant Anti-EpCAM

Abcam

ab32392

18

Anti-alpha Tubulin

Abcam

ab7291

19

Anti-Mitofusin 1

Abcam

ab57602

20

Anti-LAMP1

Abcam

ab24170

21

Anti-Cytochrome C

Abcam

ab90529

22

Anti-Parkin

Cell Signaling Technology

2132 S

23

Anti-Phospho Beclin1

Cell Signaling Technology

13825

24

Anti-COX IV

Cell Signaling Technology

4844

25

Anti-Atg5

Cell Signaling Technology

2630

26

Anti-Atg7 (D12B11) Rabbit mAb

Cell Signaling Technology

8558

27

Anti-PINK1 Rabbit pAb

ABclonal

A11435

28

Anti-Parkin Rabbit pAb

ABclonal

A0968

29

Anti-β-Actin antibody, Mouse monoclonal

Sigma

A1978-200UL

30

CD326 (EpCAM) Monoclonal Antibody (G8.8), PE

eBioscience

12-5791-82

31

Ly-6A/E (Sca-1) Antibody, PE conjugated (Mouse)

eBioscience

12-5981-82

32

CD105 Antibody PE conjugated (Mouse)

eBioscience

12-1051-82

33

CD11b Antibody FITC conjugated (Mouse)

eBioscience

11-0112-82

34

CD44 Monoclonal Antibody (IM7), PE

eBioscience

12-0441-82

35

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16)

eBioscience

14-0299

36

Rat IgG2a kappa Isotype Control (eBR2a), PE

eBioscience

12-4321-80

37

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC

eBioscience

11-4031-82

38

Mouse IgG2b kappa Isotype Control (eBMG2b), PE

eBioscience

12-4732-41

39

PE Mouse Anti-Human CD73

BD Biosciences

550257

40

PE-Cyâ„¢5 Mouse Anti-Human CD90

BD Biosciences

555597

41

FITC Mouse Anti-Human HLA-DR

BD Biosciences

555560

42

Anti-Human CD45 FITC/CD34 PE

BD Biosciences

341071

43

PE Mouse IgG1, κ Isotype Control

BD Biosciences

554080

 

Secondary Antibodies

1

Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Invitrogen

A-21206

2

Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Invitrogen

A11055

3

Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594

Invitrogen

a11037

4

Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

Invitrogen

a31573

5

Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Invitrogen

a21202

6

Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546

Invitrogen

a10036

7

Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

Invitrogen

A21247

8

Rabbit anti-mouse IgG - HRP

GeNeiTM

1140580011730

9

Rabbit anti-goat IgG – HRP

GeNeiTM

1140480011730

10

HRP-AffiniPure Goat Anti-Rabbit IgG (H + L) Secondary Antibody

Jackson ImmunoResearch Inc.

111-035-003

 

Serum

1

NORMAL GOAT SERUM

Jackson

005-000-121

2

NORMAL RABBIT SERUM

Jackson

011-000-120

 

Drugs

1

Urolithin A

Sigma

SML1791

2

Resveratrol

Sigma

R5010

3

Mito-Tempo

Santa Cruz

SC-221945

4

Pyrroloquinoline quinone

Sigma

D7783

5

Nicotinamide mononucleotide

Sigma

N3501

6

3-methyladenine

Sigma

M9281

7

N-acetyl cysteine

Sigma

A7250

8

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

Sigma

C2920

9

Oligomycin A

Sigma

75351

10

Antimycin A

Sigma

A8674

11

Rotenone

Sigma

R8875

12

Sodium Palmitate

Sigma

P9767

13

Stearic Acid

Sigma

S4751

14

Oleic acid

Sigma

O1383

 

Plasmids

1

pLYS1-FLAG-MitoGFP-HA

Addgene

50057

2

pCMV-VSV-G

Addgene

8454

3

pCMV-dR8.2 dvpr

Addgene

8455

4

pLVX-LC3-YFP

Addgene

99571

 

Cell staining dyes

1

TMRE

Invitrogen

T669

2

MitoSOX Red

Invitrogen

M36008

3

Mitotracker Red

Invitrogen

M7513

4

Mitotracker Green

Invitrogen

M7514

5

Lysotracker green

Invitrogen

L7526

6

Lysotracker deep red

Invitrogen

L12492

7

Propidium Iodide

Invitrogen

V13242

8

Hoechst 33342

Invitrogen

H21492

9

CellTraceâ„¢ CFSE Cell Proliferation Kit

Invitrogen

C34554

10

CellTrackerâ„¢ Green

Invitrogen

C2925

11

CellTrackerâ„¢ Deep Red Dye

Invitrogen

C34565

12

DAPI

Invitrogen

P36966

13

Alexa Fluorâ„¢ 594 Phalloidin

Invitrogen

A12381

14

Nonyl Acridine Orange (NAO)

Invitrogen

A1372

8

Hoest 3344

Invitrogen

H21492

9

CFSE

Invitrogen

C34554

10

Cell tracker green

Invitrogen

C2925

11

DAPI

Invitrogen

P36966

12

Phalloidin 594

Invitrogen

A12381

 

Kits

1

Senescence Cells Histochemical Staining Kit

Sigma

CS0030

2

Mitochondria Isolation Kit

Sigma

MITOISO2-1KT

3

ELISA MAXâ„¢ Deluxe Set Mouse IL-4

BioLegend

431104

4

ELISA MAXâ„¢ Deluxe Set Mouse IL-5

BioLegend

431204

5

Live Dead assay kit

Invitrogen

L3224

6

Dead cell apoptosis kit

Invitrogen

V13242

7

DeadEndâ„¢ Colorimetric TUNEL System

Promega

G7130

8

ATP assay kit

Abcam

ab83355

9

Mouse IL-13 DuoSet ELISA

R&D Systems

DY413

 

Chemicals

1

Sodium Pyruvate 100 mM

Sigma

S8636-100ML

2

Sodium Azide

Sigma

S8032-25G

3

(+)-Sodium L-Ascorbate

Sigma

A4034-100G

4

L-()-Malic Acid

Sigma

M6413-25G

5

TMPD

Sigma

T7394-5G

6

ADP

Sigma

A5285-1G

7

Succinic acid

Sigma

S9512-100G

8

Palmitoyl-L-carnitine chloride

sigma

P1645

9

TMB liquid substrate system for ELISA

Sigma

T0440-1L

10

Hydrogen peroxide–Urea adduct

Sigma

U8879-100 TAB

11

Acetyl-β-methylcholine chloride

Sigma

A2251-25G

12

Hydrogen peroxide solution

Sigma

323381-500 ML

13

Bicinchoninic Acid (BCA)

Sigma

B9643-1L

14

Water: Molecular Biology Reagent

Sigma

W4502-6X1L

15

Sodium dodecyl sulfate (SDS)

Sigma

L3771-500G

16

D-Glucose

Sigma

G8270-100G

17

Triton X 100

Sigma

T9284

18

Hexadimethrine bromide

Sigma

H9268-10G

19

Bovine Serum Albumin Fatty acid free (BSA)

Sigma

A7511

20

Insulin human

Sigma

I2643

21

Protease Inhibitor Cocktail

Sigma

p8340

22

Albumin from chicken egg white

Sigma

A5503

23

Pierceâ„¢ 16% Formaldehyde

Invitrogen

28908

24

RNA later

invitrogen

AM7021

25

cDNA synthesis kit

Invitrogen

4368814

26

LTX

Invitrogen

A12621

27

SuperSignalâ„¢ West Femto Maximum Sensitivity Substrate

Invitrogen

34095

28

SuperSignalâ„¢ West Pico PLUS Chemiluminescent Substrate

Invitrogen

34580

29

L-Glutamine (200 mM)

Invitrogen

25030081

30

HBSS

Invitrogen

14025092

31

PageRulerâ„¢ Prestained Protein Ladder

Invitrogen

26616

32

Ampicillin sodium salt

HiMedia

CMS645-5G

33

Kanamycin sulphate

HiMedia

MB105-1G

34

Phosphate Buffered Saline Tablets

Gibco

18912014

35

StemProâ„¢ MSC SFM

Gibco

A1033201

36

KAPA SYBER FAST QPCR MM

KAPA

kk4601

37

MesenCult Expansion Kit (Mouse)

Stem Cell Technologies

#05513

38

Bovine Serum Albumin (BSA)

Bio World

22070004-5

39

RNeasy Mini Kit (250)

Qiagen

74106

40

House Dust Mite Extract Dermatophagoides pteronyssinus, 2.5 ml

Greer Laboratories

XPB70D3A2.5

41

Rodent purified diet W/60% energy from Fat diet

Research Diets, Inc.

D12492

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