Fig. 5: TG2 expression in PRAD biopsies compared to controls. | Cell Death & Disease

Fig. 5: TG2 expression in PRAD biopsies compared to controls.

From: Canonical and truncated transglutaminase-2 regulate mucin-1 expression and androgen independency in prostate cancer cell lines

Fig. 5: TG2 expression in PRAD biopsies compared to controls.

Quantitative PCR of TGM2_v1 transcript (A) and TGM2_v2 transcript (B) in PRAD biopsies (n = 27) compared to CIP (n = 30). Data are mean + SEM and expressed as fold increase compared to CIP. Significant differences between PRAD grades (a) and PRAD in comparison with CIP (b) were assessed by one-way ANOVA. C Immunostaining of total TG2 protein (mouse anti-TG2 CUB7402, green), TGM2_v2 (rabbit anti-TGM2_v2, red) and nuclear staining (blue) in PRAD (n = 6) and normal adjacent tissue (NAT, n = 3) TMA (TissueArrays.com), using GeoMx platform (Nanostring Technologies). Scale bar: 0.5 mm. Box and whiskers plot shows all data points of total TG2 or TGM2_v2 intensity measured with ImageJ Fiji software and bars from min to max values. Significance was assessed by one-way ANOVA: *p ≤ 0.05, **p ≤ 0.01. The bottom panels show the detail of a grade 3 PRAD TMA acquired with a SP5 confocal microscope (63X objective; scale bars: 10 µm). White arrowheads indicate areas of TGM2_v2 nuclear localisation (pink spots).

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