Fig. 3: Conditioned media (CM) from miR-182-upregulated NSCLC cells promotes osteoclast precursor cell differentiation. | Cell Death & Disease

Fig. 3: Conditioned media (CM) from miR-182-upregulated NSCLC cells promotes osteoclast precursor cell differentiation.

From: A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer

Fig. 3: Conditioned media (CM) from miR-182-upregulated NSCLC cells promotes osteoclast precursor cell differentiation.

A–C TRAP staining analysis of the osteoclast differentiation of RAW264.7 cells after cultured in the CM from miR-182 or NC overexpressing A549 cells (A), A549-BM or parental A549 cells (B), NC or anti-miR-182-transfected A549-BM cells (C) for 6 days. Arrowheads indicate mature, multi-nucleated osteoclasts. D qPCR-based analysis of the effect of indicated CM on expression of osteoclastogenesis-related genes in RAW264.7 cells. Scale bars, 50 μm. The average values ± SEM of three separate experiments are plotted. *P < 0.05; ***P < 0.001.

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