Fig. 3: Identification of intrinsic molecules contributing to the pro-leukemic effects in MA9-IL-34 cells. | Cell Death & Disease

Fig. 3: Identification of intrinsic molecules contributing to the pro-leukemic effects in MA9-IL-34 cells.

From: Sox13 and M2-like leukemia-associated macrophages contribute to endogenous IL-34 caused accelerated progression of acute myeloid leukemia

Fig. 3

MA9-c-Kit-, MA9-c-Kit+, MA9-IL-34 cells were sorted and microarray was performed. A Venn diagram shows overlaps of DEGs obtained from each pair. B GSEA analysis shows the enrichment of genes in annotations between MA9-IL-34 and MA9-c-Kit+/MA9-c-Kit- groups. C Heat map shows the expression levels of 1572 genes, which are the DEGs between MA9-IL-34 and MA9-c-Kit+ groups, in three groups. Those genes are subdivided into 4 clusters based on the expression patterns in three groups. D The expression of selected genes was validated by qRT-PCR. E The expression level of Sox13 in the GSE10358 (n = 304), GSE61804 (n = 324) and GSE21261 (n = 76) is shown. For each dataset, AML cases were divided into IL-34low and IL-34high groups, and the corresponding expression of Sox13 is plotted. Data are presented as mean ± S.E.M. Unpaired Student’s t test, one-way ANOVA tests were used. **p < 0.01, ***p < 0.001.

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