Fig. 1: Overview of the study design. | Cell Death & Disease

Fig. 1: Overview of the study design.

From: Identification of RAC1 in promoting brain metastasis of lung adenocarcinoma using single-cell transcriptome sequencing

Fig. 1

A total 168 single cells from primary tumor tissue (PTT), primary para-tumor tissue (PTP), metastatic tumor tissue (MTT), para-metastatic tumor normal brain tissue (MTP), and circulating tumor cell (CTCs) from a LUAD patient was collected and sequenced by smart-seq2 RNA sequencing method. The data analysis was performed to find the hallmarks of metastasis related genes including RAC1. Further 7 patients with either primary tumors or metastatic tumors were collected for expansion of the single-cell data set. Pathological sections of additional six patients were further applied for RAC1 IHC staining. TCGA and survival data were also employed to find the relationship between the function of RAC1 and LUAD.

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