Fig. 6: Overexpression of EphB6 and EphrinB2 regulates soma spacing in a mutual inhibitory way.

A, B P4 cortex electroporated with control (A) and both EphB6 and EphrinB2 expression plasmid at E15.5 (B). Note that the neurons with overexpression of both EphB6 and EphrinB2 were uniformly distributed. C, D Schematic diagram showing the distribution of GFP-labeled neurons in control and Ephb6 and EphrinB2 co-transfected cortices. E, F Plot profiles indicate the fluorescence gray values in the areas labeled by dashed lines in panels (A, B). G Quantification of the intensity fluctuations in the cortical regions labeled with dashed lines in (A, B). Bar graphs are plotted as mean ± SEM, n.s., p > 0.05, Student’s t-test, n = 3 brains. H–K Mouse embryos were electroporated in utero at E15.5 with indicated combinations of two plasmids and analyzed at P4. Arrowheads indicate the clusters of neurons. L–O Fluorescence grey values were quantified from the images in (H–K), respectively, from left to right (medial to lateral in the cortex) as delineated by dashed lines. Plot profiles indicate the clustered neurons in the cortex (arrowheads in H, L and J, N). P–S Represents the distribution diagram of GFP-labeled neurons in (H–K). Scale bars, 50 μm in (A, B) and 25 μm in (H–K).