Fig. 4: LINC02428 interacts with IGF2BP1 to affect the expression of IGF2BP1 mRNA and protein levels. | Cell Death & Disease

Fig. 4: LINC02428 interacts with IGF2BP1 to affect the expression of IGF2BP1 mRNA and protein levels.

From: Downregulated liver-elevated long intergenic noncoding RNA (LINC02428) is a tumor suppressor that blocks KDM5B/IGF2BP1 positive feedback loop in hepatocellular carcinoma

Fig. 4

A FISH assay showed that LINC02428 was mainly located in the cytoplasm of HCC cells. B Western blotting of proteins obtained by RNA pull-down assay showed that LINC02428 bound to IGF2BP1. C RIP assays confirmed that LINC02428 interacts with IGF2BP1. D FISH and IF assays show LINC02428 and IGF2BP1 are co-localized in the cytoplasm in HCC cells. E Deletion mapping of the IGF2BP1-binding domain in LINC02428 was examined by western blotting. F qRT-PCR showed LINC02428 upregulation decreased IGF2BP1 mRNA levels. G Western blotting indicated LINC02428 overexpression decreased IGF2BP1 protein levels.

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