Fig. 5: LINC02428 blocked the KDM5B/IGF2BP1 positive feedback loop in HCC cells. | Cell Death & Disease

Fig. 5: LINC02428 blocked the KDM5B/IGF2BP1 positive feedback loop in HCC cells.

From: Downregulated liver-elevated long intergenic noncoding RNA (LINC02428) is a tumor suppressor that blocks KDM5B/IGF2BP1 positive feedback loop in hepatocellular carcinoma

Fig. 5

A Increased LINC02428 expression resulted in decreased KDM5B mRNA expression. B KDM5B downregulation decreased IGF2BP1 mRNA levels. C The protein lysate of KDM5B mRNA pull-down contained IGF2BP1. D RIP assays showed that IGF2BP1 could recruit KDM5B mRNA. E RNA stability assay indicated that LINC02428 overexpression could weaken the stability of KDM5B mRNA. F IGF2BP1 upregulation could reverse the LINC02428-mediated decrease in KDM5B mRNA stability. G ChIP-qPCR assay confirmed that KDM5B enriched in the promoter of IGF2BP1 and LINC02428 upregulation could decrease KDM5B enrichment. H Dual luciferase reporter assay proved that KDM5B bound to IGF2BP1 promoter to promote its transcription, and IGF2BP1 transcription promoted KDM5B could be enhanced by upregulation of IGF2BP1 or decrease by LINC02428 overexpression in 293 T. I Western blotting showed that KDM5B silencing could decrease expression of IGF2BP1 protein in HCC cells.

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