Fig. 1: Tunnelling nanotubes are present between microglia.

A HMC3 microglia stained for membrane with wheat germ agglutinin (WGA, grey) and F-Actin with phalloidin (red). Bottom panel represents the zoomed region depicted with boxes in the upper panel. Yellow arrowheads point towards TNTs. B Proportion of TNT-connected cells under normal growing conditions (N = 3 independent experiments, n = 111 regions of interests). C Lengths of the TNTs observed. D Distribution of TNTs based on their lengths. Structures less than 10 μm were excluded from our analyses. N = 3 independent experiments, n = 200 TNTs for C, D.