Fig. 3: PRMT2 increases WNT5A transcriptional expression and activates Wnt signaling.
From: PRMT2 promotes RCC tumorigenesis and metastasis via enhancing WNT5A transcriptional expression
A Volcano plot showed the differential transcripts expression by RNA-seq analysis in the ACHN-shCtrl group and the ACHN-shPRMT2 group. (Upregulated genes number: 404, whereas downregulated genes number: 396). B Gene Ontology (GO) enrichment analysis of the gene set by RNA-seq assays. Top functions are shown. C Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the gene set by RNA-seq assays. Top signaling is shown. D–G qRT-PCR assays were used to verify the results of RNA-seq in ACHN and 786-O cells after knockdown or overexpression of PRMT2. H, I Protein of WNT5A was assessed by western blots in ACHN and 786-O cell lines after overexpression (H) or knockdown (I) of PRMT2. J, K The Wnt signaling cascade-related genes (β-Catenin, c-Myc) were assessed by western blots in ACHN and 786-O cell lines after overexpression (J) or knockdown (K) of PRMT2. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
