Fig. 3: LINC00969 regulates gefitinib resistance by inhibiting the NLRP3-induced classical pyroptosis pathway. | Cell Death & Disease

Fig. 3: LINC00969 regulates gefitinib resistance by inhibiting the NLRP3-induced classical pyroptosis pathway.

From: LncRNA LINC00969 promotes acquired gefitinib resistance by epigenetically suppressing of NLRP3 at transcriptional and posttranscriptional levels to inhibit pyroptosis in lung cancer

Fig. 3: LINC00969 regulates gefitinib resistance by inhibiting the NLRP3-induced classical pyroptosis pathway.

A PC9 and PC9/GR cell morphology was observed using confocal microscopy and electron microscopy after treatment with gefitinib or LPS/ATP for 48 h. B Western blot analysis of NLRP3, pro-caspase-1, cleaved caspase-1, pro-GSDMD, and cleaved GSDMD protein levels after treatment with or without gefitinib. C After 48 h of transfection with siRNA or the overexpression plasmid, the morphology of PC9/GR cells was observed after treatment with gefitinib. D Western blot analysis of NLRP3, pro-caspase-1, cleaved caspase-1, pro-GSDMD, and cleaved GSDMD protein levels in gefitinib-treated resistant cells transfected with si-NC or si-LINC00969 after treated with gefitinib. Three independent experiments were performed. *P < 0.05, **P < 0.01; n.s. not significant.

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