Fig. 3: eIF4E expression promotes migration, invasion and EMT. | Cell Death & Disease

Fig. 3: eIF4E expression promotes migration, invasion and EMT.

From: RIPK1-dependent necroptosis promotes vasculogenic mimicry formation via eIF4E in triple-negative breast cancer

Fig. 3

A–D The wound-healing assay was conducted on SH-eIF4E MDA-MB-231, EX-eIF4E MCF-7, and control cells. E–H Migration and invasion assays were conducted on SH-eIF4E MDA-MB-231, EX-eIF4E MCF-7, and control cells. I, J The protein levels of EMT-related markers in MDA-MB-231-SH-eIF4E, MCF-7-EX-eIF4E and control cells were determined by Western blotting. (*P < 0.05, **P < 0.01, ***P < 0.001, bar = 100 μm). K, L The immunofluorescence assay results showed that eIF4E promoted the expression of EMT marker proteins (bar = 200 μm). M, N The quantitative analysis of the eIF4E-promoting EMT immunofluorescence. (**P < 0.01, ***P < 0.001) Data are representative of three independent experiments (Student’s t test).

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