Fig. 5: Overexpression of HK2 hinders I/R-induced kidney injury in HIF-1α C-TAD−/− mice. | Cell Death & Disease

Fig. 5: Overexpression of HK2 hinders I/R-induced kidney injury in HIF-1α C-TAD−/− mice.

From: Activation of HIF-1α C-terminal transactivation domain protects against hypoxia-induced kidney injury through hexokinase 2-mediated mitophagy

Fig. 5

a Scr and BUN level in HIF-1α CTAD−/− mice subjected to 35 min of I/RI following Lv-HK2 administration (n = 6). b qRT-PCR analysis of KIM-1 expression in HIF-1α CTAD−/− mice subjected to 35 min of I/RI following Lv-HK2 administration. The relative levels were normalized to β-actin (n = 6). c Representative images of KIM-1 expression in kidney tissues were assessed by immunohistochemistry (n = 6). Scale bars, 50 µm. d Representative histological changes (PAS staining) (left, n = 6). Scale bars, 100 µm. The quantification of tubular injury based on PAS staining (right, n = 6). e mRNA expression of inflammatory factors (MCP-1, TNF-α, and IL-1β) in kidney tissues from HIF-1α CTAD−/− mice injected with Lv-HK2 (n = 6). f. Immunohistochemistry and semiquantitative population of F4/80+ macrophages infiltrating the kidneys (n = 6). Scale bars, 100 µm. **p-value < 0.01 versus Lv-NC. Data are presented as mean ± SEM of 6 mice. Lv-NC served as the control (t-test).

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