Fig. 5: Lipid droplets but not free cholesterol associate with autophagosome. | Cell Death & Disease

Fig. 5: Lipid droplets but not free cholesterol associate with autophagosome.

From: Autophagy regulates sex steroid hormone synthesis through lysosomal degradation of lipid droplets in human ovary and testis

Fig. 5: Lipid droplets but not free cholesterol associate with autophagosome.The alternative text for this image may have been generated using AI.

A Representative confocal images of the luteinized granulosa cells triple stained for LAMP2 (green signal), perilipin3 (yellow signal) and LC3 (red signal) following treatment with hCG (10 IU/ml) w/wo chloroquine (CQ, 60 μM). Quantification of the signal intensities and co-localizations of the signals are indicated to the right of the images. Nuclei stained with DAPI. Scale bars represent 20 μm. Mean ± SD, N = 3 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons. B Representative confocal images of the luteinized granulosa cells double-stained for Filipin (blue signal) and LC3B (green signal) following treatment with hCG (10 IU/ml) w/wo chloroquine (CQ, 60 μM). Quantification of the signal intensities and co-localizations of the signals are indicated to the right of the images. Nuclei stained with DRAQ5 (red signal). Scale bars represent 20 μm. Mean ± SD, N = 3 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons.

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