Fig. 3: Ablation of Mettl3 causes infertility due to compromised uterine receptivity and decidualization. | Cell Death & Disease

Fig. 3: Ablation of Mettl3 causes infertility due to compromised uterine receptivity and decidualization.

From: METTL3-dependent m6A methylation facilitates uterine receptivity and female fertility via balancing estrogen and progesterone signaling

Fig. 3: Ablation of Mettl3 causes infertility due to compromised uterine receptivity and decidualization.The alternative text for this image may have been generated using AI.

Representative immunofluorescence images (A) and the percentage (B) of Ki67+ epithelial cells and stromal cells in uterine of Mettl3 cKO and control mice on GD4. Data were calculated using 11 images from 3 control mice and 14 images from 3 Mettl3 cKO mice. Representative immunofluorescence images (C) and quantification of E-cadherin (D) in uterine epithelial cells of Mettl3 cKO and control mice on GD4. Fluorescence intensities of uterine epithelial E-cadherin were calculated using 6 images from 3 control mice and 12 images from 3 Mettl3 cKO mice. Representative immunofluorescence images (E) and the percentage (F) of Ki67+ epithelial cells and stromal cells in uterine of Mettl3 cKO and control mice following induction of artificial pregnancy (pollard experiment). Data were calculated using 12 images from 3 control mice and 7 images from 3 Mettl3 cKO mice. Representative immunofluorescence images (G) and quantification of E-cadherin (H) in uterine epithelial cells of Mettl3 cKO and control mice following induction of artificial pregnancy. Fluorescence intensities of uterine epithelial E-cadherin were calculated using 12 images from 3 control mice and 15 images from 3 Mettl3 cKO mice. A, C, E, G Nuclei were stained with DAPI. Scale bars: 75 μm. I Immunohistochemistry of METTL3 with paraffin sections from pregnant females on GD0 and GD5. Sections are counterstained with hematoxylin. Brown staining denotes METTL3+ cells. Scale bars: 100 μm. J Representative pictures showing the gross morphology of oil-treated uterine horns (left horn) and untreated uterine horns (right horn) from control (n = 6) and Mettl3 cKO mice (n = 6) collected 5 days after oil injection. Scale bars: 1 cm. K The weight per unit length of oil-injected and untreated uterine horns in (J). Data are presented as mean ± SD, **P < 0.01. L Histology of control and Mettl3 cKO mice uterus in (J), as measured by HE staining, Scale bars 200 μm. M Relative mRNA levels of decidualization marker genes (Wnt4, Bmp8a, Bmp2, Prl8a2) in the uterine horns of control and Mettl3 cKO mice in (J). B, D, F, H, M Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, relative to control and unstimulated.

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