Fig. 2: C3 is required for PTX resistance in A549 cells. | Cell Death & Disease

Fig. 2: C3 is required for PTX resistance in A549 cells.

From: Nuclear complement C3b promotes paclitaxel resistance by assembling the SIN3A/HDAC1/2 complex in non-small cell lung cancer

Fig. 2: C3 is required for PTX resistance in A549 cells.The alternative text for this image may have been generated using AI.

A C3 insufficiency enhanced PTX cytotoxicity in A549-PTX cells, as demonstrated by the CCK8 assay. Data represent the mean ± SD, n = 5, two-tailed Student’s t test. C3 insufficiency reduced the colony formation ratio of A549-PTX cells with PTX treatment. Images (B) and quantitative results (C). Data represent the mean ± SD, n = 3, two-tailed Student’s t test. C3 insufficiency promoted PTX-induced apoptosis in A549-PTX cells, as detected by FCM analysis. Scatter plot (D) and quantitative results (E). FCM, flow cytometry. Data represent the mean ± SD, n = 3, two-tailed Student’s t test. F–H C3 insufficiency increased the sensitivity of A549-PTX cells to PTX treatment in tumor-bearing mice. Images (D), growth curve (E) and tumor weight (F). Data represent the mean ± SEM, n = 5, two-tailed Student’s t test. I Ectopic C3 expression impeded PTX cytotoxicity in A549-ORI cells, as demonstrated by the CCK8 assay. Data represent the mean ± SD, n = 5, two-tailed Student’s t test. J–K Ectopic C3 expression suppressed PTX-induced apoptosis in A549-ORI cells, as detected by FCM analysis. Data represent the mean± SD, n = 3, two-tailed Student’s t test. ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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