Fig. 7: USP33 was transcriptional activated by ZEB1. | Cell Death & Disease

Fig. 7: USP33 was transcriptional activated by ZEB1.

From: USP33 promotes pancreatic cancer malignant phenotype through the regulation of TGFBR2/TGFβ signaling pathway

Fig. 7

A The expression of ZEB1 in PC cells transfected with ZEB1 shRNA or overexpression plasmids. B The expression of USP33 mRNA in PC cells transfected with ZEB1 shRNA or overexpression plasmids. C The expression of USP33 protein in PC cells transfected with ZEB1 shRNA or overexpression plasmids. D, E The motif of ZEB1 predicted by JASPAR database. F The possible regions in USP33 promoter for the binding of ZEB1. G, H The dual-luciferase reporter assay revealed the binding between indicated regions of USP33 promoter and ZEB1. I, J The ChIP-qPCR assay revealed the binding between indicated regions of USP33 promoter and ZEB1. K, L The CCK-8 assay in PC cells co-transfected with USP33 overexpression plasmids and ZEB1 shRNA. M The quantification of EdU assay in PC cells co-transfected with USP33 overexpression plasmids and ZEB1 shRNA. N The quantification of colony formation assay in PC cells co-transfected with USP33 overexpression plasmids and ZEB1 shRNA. O, P The quantification of Transwell assay in PC cells co-transfected with USP33 overexpression plasmids and ZEB1 shRNA.

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