Fig. 5: KLF6 overexpression attenuates H/R‑induced AML12 cell injury.
From: KLF6 alleviates hepatic ischemia-reperfusion injury by inhibiting autophagy
a, b Western blotting and RT-qPCR analysis of KLF6 expression of Vector and KLF6 cells. c The viability of Vector and KLF6 cells following hypoxia and reoxygenation was evaluated by a CCK8 assay at the indicated time points. d Western blotting analysis for cleaved caspase3, BAX and Bcl2 after H/R in the Vector and KLF6 cells. e Upper panel, representative flow cytometry plots of annexin V/PI stained cells. Lower panel, statistical analysis of total apoptotic cells in different groups. f Representative pictures (upper panel) and quantitative analysis (lower panel) of nucleus stained with Hoechst 33342. Scale bar, 200 μm. g In situ fluorescent staining (red) detection of ROS in Vector and KLF6 cells after H/R (upper panel) and quantified average fluorescence intensity by ImageJ (lower panel). Scale bar, 100 μm. Vector AML12 cells were infected with an empty control lentiviral vector, KLF6 AML12 cells were infected with a lentiviral construct expressing KLF6. Mean values ± SD are shown, and statistical significance was determined by two-tailed Student’s t test. *p < 0.05.
