Fig. 9: Th17 cells promote HCC recurrence in vivo.

A Liver tissue images of mice with HCC recurrence and liver index (liver weight/body weight), n = 4–5, scale bars, 1 cm. B H&E staining to observe the morphology of recurrent tumors and hepatic replacement area (HRA) of HCC recurrence, n = 4–5. C, D, F The mRNA levels of cancer stemness indicators (C), pre-metastasis microenvironment indicators (D), and EMT indicators (E) in mice models. F Western blotting analysis of EMT indicators, cancer stemness indicators, and pre-metastasis microenvironment indicators in mice models. G Quantization of Western analysis. mean ± SEM, *p < 0.05, **p < 0.01, Student t-test.