Fig. 3: DHT interacts with the EPPK1 protein in bladder cancer. | Cell Death & Disease

Fig. 3: DHT interacts with the EPPK1 protein in bladder cancer.

From: Androgen dihydrotestosterone promotes bladder cancer cell proliferation and invasion via EPPK1-mediated MAPK/JUP signalling

Fig. 3

A Structural formula of biotinylated DHT. B Biotinylated-DHT pull-down assay. Expression of the top 20 candidate proteins by mass spectrometry was presented using a heatmap after biotinylated-DHT pull-down assays in T24 cells. C EPPK1 was more highly expressed in five-six cancer tissues than in normal tissues, as measured by qRT‒PCR. D EPPK1 protein was more highly expressed in MIBC (score = 6.80 ± 3.809) than in NMIBC (4.90 ± 3.398) (p = 0.046) and paired paracancerous tissues (2.517 ± 2.626) (p < 0.001). E The proliferation and invasive abilities were decreased in cells with EPPK1 knockdown. F Genetic sequencing of EPPK1 in six male and six female patients exhibiting EPPK1 mutations was common in BLCA, five mutation sites and their positions on chromosomes can be detected in more than eight samples was shown. Scale bar = 100 μm. *p < 0.05.

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