Fig. 2: SHP2 phosphatase inhibits Src/OTUB1/Raptor axis by ODN. | Cell Death & Disease

Fig. 2: SHP2 phosphatase inhibits Src/OTUB1/Raptor axis by ODN.

From: Cathepsin K inhibition induces Raptor destabilization and mitochondrial dysfunction via Syk/SHP2/Src/OTUB1 axis-mediated signaling

Fig. 2

A Caki-1 cells were transfected with Cont siRNA or 41 tyrosine phosphatases siRNA followed by treatment with 2 μM ODN for 24 h. B Cancer cells (Caki-1, A549, and DU145) were transfected with Cont siRNA or SHP2 siRNA for 24 h. C Cancer cells (Caki-1, A549, and DU145) were treated with 2 μM ODN for the indicated periods. D Cancer cells (Caki-1, A549, and DU145) were transfected with Cont siRNA or Cat K siRNA for 24 h. E Cancer cells were pretreated with 10 μM SHP099 for 30 min, followed by treatment with 2 μM ODN for 24 h. F Caki-1 cells were transfected with Cont siRNA or SHP2 siRNA and followed by treatment with 2 μM ODN for 24 h. G Caki-1 were transiently co-transfected with a vector, SHP2 wild-type (WT), or SHP2 mutant (C459S) with Src WT or Src mutant (K295R/Y527F). Protein expression was determined using western blotting. The band intensity of Raptor protein was quantified by using ImageJ (A).

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