Fig. 6: RNF128 interacts with TLR4 and enhances its ubiquitination.
From: RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury
A Interaction between endogenous RNF128 and TLR4. Extracts from MH-S cells were immunoprecipitated using anti-RNF128, anti-TLR4 or rabbit anti-immunoglobulin G (anti-IgG) antibodies and analysed using anti-RNF128 and anti-TLR4 antibodies. B, C The subcellular localisation of RNF128 and TLR4 in MH-S or primary alveolar macrophages was examined using immunofluorescence microscopy (THUNDER Imaging Systems). E, F Lysates from MH-S (RNF128 overexpression or silencing) stable cell lines were extracted, immunoprecipitated (IP) using TLR4 antibody, and analysed for ubiquitylation using the specified antibody. D, G, H HEK293 cells were transiently transfected with HA-Ubiquitin (Ub), HA-Ub-K48, TLR4, RNF128 and RNF128 (H2N2) after 24 h. Harvested lysates were immunoprecipitated using TLR4 antibody and analysed for ubiquitylation after immunoblotting.
