Fig. 7: RNF128 suppresses LPS-induced NF-κB activation and pro-inflammatory cytokine expression in MH-S cells.
From: RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury
A Immunoblots of NF-κB and p-NF-κB in MH-S/shluc and MH-S/shRNF128 cell lines treated with LPS (100 ng/ml) for 2 h. Each lane showed the intensity ratio of NF-κB and p-NF-κB. The results shown are representative of at least three independent experiments. B Immunoblots of NF-κB and p-NF-κB in MH-S/vector and MH-S/RNF128 cell lines treated with LPS (100 ng/ml) for 2 h. Each lane showed the intensity ratio of NF-κB and p-NF-κB. The results shown are representative of at least three independent experiments. C The expression of TNF-α, IL-1β and IL-6 mRNAs in MH-S/shluc and MH-S/shRNF128 cell lines treated with or without LPS (100 ng/ml) for 2 h. D The expression of TNF-α, IL-1β and IL-6 mRNAs in MH-S/vector and MH-S/RNF128 cell lines treated with or without LPS (100 ng/ml) for 2 h. E The protein levels of IL-1β, and IL-6 in MH-S/shluc and MH-S/shRNF128 cell lines treated with or without LPS (100 ng/ml) for 2 h. F The protein levels of IL-1β, and IL-6 in MH-S/vector and MH-S/RNF128 cell lines treated with or without LPS (100 ng/ml) for 2 h. The data are presented as mean ± SD. The statistical significance was evaluated using one-way ANOVA with Newman–Keuls post-hoc test or Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.
