Fig. 7: FBXO7 suppresses INF2-DRP1 axis-associated mitochondrial division.

A Representative images of U-2OS cells with FBXO7 knockout or parental transfected with pCDH-CD513B-FBXO7-WT/D72G plasmids, siINF2 RNA, siDNM1L RNA, or treated with Mdivi-1 (20 μM). stained with INF2, DRP1, Mito-Tracker Red and DAPI. Scale bar, 20 μm. B Quantification of mitochondria lengths in (A). Data are shown as means ± SD (n = 20). *p < 0.05, ***p < 0.001. C Western blotting of WCLs of U-2OS cells in (A). The quantitation except DRP1 Ser616 and DRP1 Ser637 were normalized to the protein level of endogenous parental GAPDH. The quantitation of DRP1 Ser616 and DRP1 Ser637 phosphorylation were normalized to the protein level of endogenous parental DRP1. D Statistics of the ratio between DRP1 Ser616 to DRP1 Ser637. Data are shown as means ± SD (n = 3). *p < 0.05. E Quantification of DRP1 puncta in (A). Data are shown as means ± SD (n = 20). *p < 0.05, ***p < 0.001. F Representative images (Left) and staining patterns (Right) of IHC analysis of DRP1 Ser616 and DRP1 Ser637 expression on ECa (n = 20) and corresponding normal endometrium tissues. Scale bar, 100 μm. *p < 0.001 vs. the Normal group. Experiments in (C) were repeated three times.