Fig. 2: NK1R is a cell surface receptor co-upregulated with NE markers and NE-related genes in tNEPC. | Cell Death & Disease

Fig. 2: NK1R is a cell surface receptor co-upregulated with NE markers and NE-related genes in tNEPC.

From: Neurokinin-1 receptor drives PKCɑ-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer

Fig. 2

A Comparison of NK1R expression in human CRPCs (n = 34) and NEPCs (n = 14) from the dataset of Beltran H et al. 2016 or in human prostate adenocarcinomas (n = 20) and NEPCs (n = 4) (GSE66187). B NK1R expression in benign prostate tissues (n = 6), localized primary prostate cancer tissues (n = 7), and metastatic prostate cancer tissues (n = 6) (GSE3325). C Protein level of CgA, ENO2, AR, PSA, and NK1R in a panel of prostate cancer cell lines. D Cell survival of 22Rv1-NE, LNCaP-NE, and their parental cells treated with enzalutamide for 72 h. E Protein level of NK1R is upregulated in enzalutamide-resistant 22Rv1 and LNCaP cells compared to parental 22Rv1 and LNCaP cells. F Relative mRNA level of NE-related genes and AR target genes in 22Rv1-NE, LNCaP-NE, and their parental cells. G FACS analysis and quantification of NK1R expression on the cell surface of 22Rv1, 22Rv1-NE, LNCaP, and LNCaP-NE cells. H Protein level analysis of NK1R, AR, PSA, and CgA in sub-colonies derived from 22Rv1-NE and LNCaP-NE cells. Data was presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.

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