Fig. 4: TIMM13 depletion inhibits OS cell proliferation and migration.
From: HOXC13-driven TIMM13 overexpression promotes osteosarcoma cell growth
The primary OS cells, pOS-1, expressing the TIMM13 shRNA (“shTIMM13-S1”/“shTIMM13-S2”, with different sequences), the CRISPR/Cas9–TIMM13–KO plasmid (“koTIMM13”), or the scramble control shRNA plus the Cas9 empty vector (“shC+koC”), were established and cultivated under the complete medium for designated hours; cell proliferation, viability, and migration were examined by EdU staining (A), CCK-8 (B), and “Transwell” (C) assays, respectively. The primary OS cells, pOS-2, or the immortalized MG63 cells, with shTIMM13-S1 (“shTIMM13”) or the scramble control shRNA (“shC”), were cultivated under the complete medium for designated hours; cell proliferation (D) and migration (E) were tested similarly, with results quantified. “Pare” stands for the parental control of OS cells. Error bars stand for mean ± standard deviation (SD, n = 5). *P < 0.05 versus “Pare”/“shC” cells. Experiments in this figure were repeated five times. Scale bar = 100 μm.
