Fig. 5: Ectopic TIMM13 overexpression further promotes OS cell proliferation and migration.
From: HOXC13-driven TIMM13 overexpression promotes osteosarcoma cell growth
pOS-1 primary cells, expressing the GV248-TIMM13-GFP-expressing construct (TIMM13OE-S1/S2, two stable selections) or the GV248 empty vector (“Vec”), were established, and expression of listed mRNAs and proteins was shown (A, B), with the cellular ATP contents examined (C). Cell proliferation and migration were examined by EdU staining (D) and “Transwell” (E) assays, respectively. The primary OS cells, pOS-2, or the immortalized MG63 cells, with TIMM13-expressing construct (“TIMM13OE”) or the empty vector (“Vec”), were established, TIMM13 and TIMM8a mRNA expression were tested by qRT-PCR assays (F, G); cells were further cultivated under the complete medium for designated hours; ATP contents (H), cell proliferation (by measuring EdU-positive nuclei ratio, I and migration (by measuring migrated cell number, J were tested similarly. Error bars stand for mean ± standard deviation (SD, n = 5). *P < 0.05 vs. “Vec” cells. Experiments in this figure were repeated five times. Scale bar = 100 μm (D, E).
