Fig. 6: S100A4 mediates the functions of STC1 in breast cancer lung metastasis.
From: Stanniocalcin 1 promotes lung metastasis of breast cancer by enhancing EGFR–ERK–S100A4 signaling
A Validation of S100A4 knockdown and overexpression in human breast cancer cell lines, MDA-MB-231-STC1 and LM2-shSTC1, by Western blot analyses. B Transwell invasion of MDA-MB-231(EV, STC1, and STC1-shS100A4) and LM2 (shNC, shSTC1, and shSTC1-S100A4). C Tube formation of HUVECs co-cultured with MDA-MB-231 (EV, STC1, and STC1-shS100A4) or LM2 (shNC, shSTC1, and shSTC1-S100A4). D Transwell migration of MRC5 co-cultured with MDA-MB-231 (EV, STC1, and STC1-shS100A4) or LM2 (shNC, shSTC1, and shSTC1-S100A4). E Lung metastasis nodule quantification and representative images after tail vein injection of MDA-MB-231 (EV, STC1, and STC1-shS100A4) or LM2 (shNC, shSTC1, and shSTC1-S100A4); n = 6 mice per group. The p values were obtained by one-way ANOVA test with Tukey’s multiple comparisons test. Scale bars, 100 μm. STC1 stanniocalcin 1. **p < 0.01; ***p < 0.001.
